Cat#:ADV-1006EK;Product Name:Human Folic acid(FA) ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma;Description:This Human Folic acid (FA) ELISA Kit was designed for the quantitative measurement of Human Folic acid (FA) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 12.5 pg/mL-200 pg/mL and the sensitivity is 6.25 pg/mL.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human FA antibody. Standard (10 x concentrate)(1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve. HRP-conjugated FA (100 x concentrate) (1 x 6oμl) HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated FA antibody solution. Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration. Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances. TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:12.5 pg/mL-200 pg/mL;Sensitivity:6.25 pg/mL;
This Human Folic acid (FA) ELISA Kit was designed for the quantitative measurement of Human Folic acid (FA) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 12.5 pg/mL-200 pg/mL and the sensitivity is 6.25 pg/mL.
Species Reactivity:
Human
Usage:
For Research Use Only
Detection Principle:
Competitive ELISA
Contents of Kit:
A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human FA antibody. Standard (10 x concentrate)(1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve. HRP-conjugated FA (100 x concentrate) (1 x 6oμl) HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated FA antibody solution. Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration. Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances. TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual