Cat#:ADV-1049EK;Product Name:Human Estradiol, E2 ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma.;Description:The human Estradiol (E2) ELISA kit is a powerful tool for quantitative measurement of estradiol levels in human serum and plasma samples, with a detection range of 50 pg/ml to 1200 pg/ml and a sensitivity of 40 pg/ml. The assay principle is competitive, meaning that two antibodies competitively bind to the estradiol in the sample. The detection is read at a wavelength of 450 nm. The assay time typically ranges from 1 to 5 hours, requiring a sample volume of 50-100 µl. The kit's performance has been validated in multiple studies found in more than 18 publications, ensuring its accuracy and reproducibility in measuring estradiol levels in human samples.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody. Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve. Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody. HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction. Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances. Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color. Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color. Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:50 pg/ml-1200 pg/ml.;Sensitivity:40 pg/ml.;