Cat#:ADV-1361EK;Product Name:Human Arachidonic Acid, AA ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Cell Culture Supernates, Tissue Homogenates;Description:This human arachidonic acid (AA) ELISA Kit is suitable for qualitatively determining human arachidonic acid concentrations in serum, plasma, cell culture supernates, or tissue homogenates in vitro. Arachidonic acid participates in important biological processes such as growth, and development, and is a precursor of numerous lipid mediators. When obtained from food such as poultry, animal organs & meat, fish, seafood, and eggs, arachidonic acid is incorporated in phospholipids in the cells' cytosol, adjacent to the endoplasmic reticulum membrane that is studded with the proteins necessary for phospholipid synthesis and their allocation to the diverse biological membranes. Arachidonic acid can also be obtained by desaturation and chain elongation of the plant-rich essential fatty acid, linoleic acid. This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with an antibody specific to AA. Standards or samples are added to the appropriate microtiter plate wells with an HRP-conjugated AA. The competitive inhibition reaction is launched between HRP-conjugated AA and AA in samples. A substrate solution is added to the wells and the color develops oppositely to the amount of AA in the sample. The color development is stopped and the intensity of the color is measured.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Detection Range:78.125 ng/mL-5000 ng/mL;Sensitivity:69.748 ng/mL;