Cat#:ADV-0468EK;Product Name:Fish immunoglobulin M (IgM) ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Tissue Homogenates;Description:This Fish IgM ELISA Kit was designed for the quantitative measurement of Fish IgM protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.25 μg/mL-50 μg/mL and the sensitivity is 1.25 μg/mL.;Species Reactivity:Fish;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate --The 96-well plate has been pre-coated with goat-anti-rabbit antibody. Standard (6 x 0.5 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve. HRP-conjugated IgM (1 x 6 ml) --Bind to the IgM antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction. IgM Antibody (1 x 6 ml) --Interact with the IgM. Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration. Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances. TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:1.25 μg/mL-50 μg/mL;Sensitivity:1.25 μg/mL;