Cat#:ADV-0815EK;Product Name:Chicken Immunoglobulin A, IgA ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma;Description:The Chicken Immunoglobulin A (IgA) ELISA Kit can be your reliable solution for measuring immunoglobulin A in Gallus gallus (chicken) samples. This ELISA kit is designed to detect and quantify IgA protein in serum and plasma samples with a wide detection range of 781.25 ng/mL-50000 ng/mL and a sensitivity of 390 ng/mL. This kit utilizes a competitive measurement principle, making it a highly quantitative assay for researchers in the field of immunology. The assay time is quick and efficient, taking only 1-5 hours to complete, and requires a small sample volume of 50-100ul. The detection wavelength of 450 nm ensures high accuracy and precision in your measurements. With over 17 citations, our Chicken Immunoglobulin A (IgA) ELISA Kit has been tested and validated by numerous researchers, providing accurate and reliable results in their studies. It is an essential tool for researchers studying the immune system of chickens, allowing them to better understand the mechanisms and functions of IgA protein in this species.;Species Reactivity:Chicken;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate --The 96-well plate has been pre-coated with chicken IgA. Standard(10 x concentrate) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve. HRP-conjugated IgA antibody(100 x concentrate) (1 x 60 μl) --Bind to the IgA, and HRP catalyzes the TMB to elicit a chromogenic reaction. HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated IgA antibody solution. Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration. Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances. TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:781.25 ng/mL-50000 ng/mL;Sensitivity:390 ng/mL;