Cat#:EIA-764C;Product Name:Bovine ALB ELISA Kit;Size:96T;Sample:serum, plasma, milk, colostrum, and cell culture supernatant;Intended use:RUO;Synonym:ALB; albumin; MGC179277; serum albumin; BSA; EC 6.1.1.16;Background:Albumin is the most predominant protein in serum of domestic animals and man. It serves several functions including the binding and transporting of fatty acids, hormones, and metal ions, the maintenance of osmotic pressure and pH, and binding of exogenous toxins and products of lipid oxidation (Bertucci, 2002). Bovine and human albumins are carbohydrate-free monomeric proteins composed of three homologous domains (Peters, 1985 and Sugio, 1999). Over time, development of large-scale purification methods of bovine albumin have translated those functions into diagnostic, cell culture, and microbiological applications, (Mannuzza 2002) allowing for numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Bovine serum albumin is also used as a nutrient in cell and microbial culture.;Species Reactivity:Bovine;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;
serum, plasma, milk, colostrum, and cell culture supernatant
Intended use:
RUO
Gene Introduction:
Albumin is the most predominant protein in serum of domestic animals and man. It serves several functions including the binding and transporting of fatty acids, hormones, and metal ions, the maintenance of osmotic pressure and pH, and binding of exogenous toxins and products of lipid oxidation (Bertucci, 2002). Bovine and human albumins are carbohydrate-free monomeric proteins composed of three homologous domains (Peters, 1985 and Sugio, 1999). Over time, development of large-scale purification methods of bovine albumin have translated those functions into diagnostic, cell culture, and microbiological applications, (Mannuzza 2002) allowing for numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Bovine serum albumin is also used as a nutrient in cell and microbial culture.
Species Reactivity:
Bovine
Application:
quantitate
Usage:
For Lab Research Use Only
Detection Principle:
Sandwich ELISA
Storage:
Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.