Cat#:EIA-465C;Product Name:Human Interleukin 8, IL8 ELISA Kit;Size:96T;Sample:serum, plasma and other biological fluids;Intended use:RUO;Synonym:IL8; interleukin 8; CXCL8; GCP-1; GCP1; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; K60; T cell chemotactic factor; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; lymphocyte-derived neutrophil-activating factor; emoctakin; granulocyte chemotactic protein 1; monocyte-derived neutrophil chemotactic factor; neutrophil-activating peptide 1; T-cell chemotactic factor; small inducible cytokine subfamily B, member 8; b-ENAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; Neutrophil-activating protein 1;Background:Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. There are more receptors of the surface membrane capable to bind IL-8; the most frequently studied types are the G protein coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity to IL-8 is different in the two receptors (CXCR1 > CXCR2). Toll-like receptors are the receptors of the innate immune system. These receptors recognize antigen patterns (like LPS in gram negative bacteria). Through a chain of biochemical reactions IL-8 is secreted and is an important mediator of the immune reaction in the innate immune system response.;Species Reactivity:Human;Application:Quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;