Cat#:EIA-227C;Product Name:Phage ELISA Kit;Size:96 T;Sample:N/A;Intended use:RUO;Synonym:Bacteriophage; Phage; M13; M13 Bacteriophage;Background:The phage display technology is a powerful and well-established tool for the investigation of proteinprotein interactions and for the generation of human antibodies for in vitro diagnostics and in vivo therapy. Generally several rounds of selection on an immobilized antigen are required to enrich and isolate the binding phage. The need to determine phage titers before and after each round to monitor the panning process still represents a major time and material consuming factor within this procedure. Usually, the phages are titered by infecting E. coli with serial dilutions of phage, plating the bacteria on agar plates and counting the colony (cfu) or plaque forming units (pfu) after over-night incubation. To determine the relative number of phage particles.;Species Reactivity:Virus;Application:Quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwish ELISA;