Cat#:RP-6068H;Product Name:Recombinant Human TGF b 1 Protein, CHO;Synonym:Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.;Description:TGFB1 Protein produced in CHO cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa.?;Source:Chinese Hamster Ovary cells.;AA Sequence:ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS;Purity:Greater than 95% as determined by SDS-PAGE.;Bioactivity:The ED50 for this effect is typically 20-60ng/ml before activation and 0.1-1ng/ml after activation as measured by TGF-b1’s ability to inhibit the mouse IL-4-dependent proliferation of HT-2 cells. Activation: Activated in vitro at pH below 3.5 and over 12.;Formulation:The TGF b 1 Human 0.22 µm filtered solution (0.24mg/ml) contains 20mM TRIS HCl buffer pH 7.2.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks.? Store, frozen at -20°C for longer periods of time.? For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;