Cat#: | RP-6064H |
Product Name: | Recombinant Human TGF b 1 Protein |
Synonym: | Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1. |
Description: | Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa. The TGF-b 1 is purified by proprietary chromatographic techniques. |
Source: | Human Platelets. |
Purity: | Greater than 98.0% as determined by SDS-PAGE. |
Bioactivity: | Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5ng/ml corresponding to a specific activity of 200,000-10,000,000IU/mg. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF- must |
Formulation: | TGF-Beta1 protein was lyophilized from a solution containing 30% acetonitrile and 0.1% trifluoroacetic acid. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute lyophilized TGF-beta 1 in 0.5% BSA in 0.1N acetic acid, which can then be further diluted to the desired aliquot with 30% acetonitrile and 0.1% trifluoroacetic acid. |
Storage: | Lyophilized TGF-beta 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGF-beta 1 should be stored at 4°C between 2-7 days and for future use below -18°C. DO NOT RECONSTITE WITH NEUTRAL BUFFERS. DO NOT USE GLASS IMPLEMENTS OR EXTENSIVE MANIPULATIONS. PREVENT FREEZE THAW CYCLES. |