Cat#:RP-6065H;Product Name:Recombinant Human TGF b 1 Protein Recombinant;Synonym:Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.;Description:TGFB1 Protein produced in Human 293 cells is a glycosylated homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25.6kDa. The TGFB1 is purified by proprietary chromatographic techniques.;Source:Human 293 cells.;AA Sequence:ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:The ED50 as determined by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells is less than 0.05ng/ml, corresponding to a specific activity of 2x107units/mg.;Formulation:Lyophilized from a sterile filtered solution containing 50mM Glycine, 50mM sodium chloride, 10% Trehalose and BSA (4:1 protein to BSA ratio) pH 4.0.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized TGFB1 in sterile 10mM Acetic Acid at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;