Cat#:RP-6074H;Product Name:Recombinant Human TGF b 3 Protein, Plant;Synonym:Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.;Description:TGFB3 Protein produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2kDa. The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.;Source:Nicotiana benthamiana.;AA Sequence:HHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKG YYANFCSGPCPYLRSADTTHSTVLGLY NTLNPEASASP CCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS.;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:The biological activity of TGFB3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 ? 40ng/ml corresponding to a specific activity of 25,000 Units/mg.;Formulation:Lyophilized from a concentrated (1mg/ml) solution containing 50mM Tris-HCl pH-7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized TGFB3 in sterile 5mM HCl & 50ug/ml BSA at a concentration of 0.05mg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized TGFB3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB3 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;