Cat#: | RP-4020H |
Product Name: | Recombinant Human IL12 p40 Protein, Plant |
Synonym: | NKSF2, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), TSF, Edodekin-alpha, IL-12 p40, IL-12B, IL-12 subunit p40, NK cell stimulatory factor chain 2. |
Description: | IL-12 p40 Protein produced in Nicotiana benthamiana plant is a glycosilated polypeptide chain containing 312 amino acids, 23-328 aa (molecular formula C1575H2420N424O490S12). IL-12 p40 is fused to a 6-His-tag at the N-terminal having the total molecular mass of 35.5kDa however as a result of potential glycosylation, the molecular mass could be approximately 40kDa by SDS PAGE under reducing conditions. IL-12 p40 IS purified by standard chromatographic techniques. |
Source: | Nicotiana benthamiana plant |
AA Sequence: | HHHHHHIWEL KKDVYVVELD WYPDAPGEMV VLTCDTPEED GITWTLDQSS EVLGSGKTLT IQVKEFGDAG QYTCHKGGEV LSHSLLLLHK KEDGIWSTDI LKDQKEPKNK TFLRCEAKNY SGRFTCWWLT TISTDLTFSV KSSRGSSDPQ GVTCGAATLS AERVRGDNKE YEYSVECQED SACPAAEESL PIEVMVDAVH KLKYENYTSS FFIRDIIKPD PPKNLQLKPL KN |
Purity: | Greater than 97.0% as determined by Analysis by SDS-PAGE. |
Formulation: | Lyophilized from 1mg/ml solution in 20 mM PBS buffer pH 7.4. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized IL-12 p40 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage: | Lyophilized IL-12 p40 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-12 p40 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |