Cat#:RP-1653M;Product Name:Recombinant Mouse AKR1B1 Protein;Synonym:Aldose reductase, AKR1B1, AR, Aldehyde reductase, Akr1b3, Aldor1, Aldr1, Akr1b1, Ahr-1, Ahr1, ALR2.;Description:AKR1B1 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-316a.a.) and having a molecular mass of 38.1kDa. AKR1B1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.;Source:E.coli.;AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSMASHLEL NNGTKMPTLG LGTWKSPPGQ VTEAVKVAID LGYRHIDCAQ VYQNEKEVGV ALQEKLKEQV VKRQDLFIVS KLWCTFHDKS MVKGAFQKTL SDLQLDYLDL YLIHWPTGFK PGPDYFPLDA SGNVIPSDTD FVDTWTAMEQ LVDEGLVKTI GVSNFNPLQI ERILNKPGLK YKPAVNQIEC HPYLTQEKLI EYCHSKGIVV TA;Purity:Greater than 95% as determined by SDS-PAGE.;Bioactivity:Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.;Formulation:AKR1B1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4), and 10% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;
AKR1B1 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-316a.a.) and having a molecular mass of 38.1kDa. AKR1B1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
Formulation:
AKR1B1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4), and 10% glycerol.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Storage:
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.