Cat#: | RPH-NP132 |
Product Name: | Recombinant Human Fibroblast Growth Factor 2 / FGF2 / FGFb Protein(Gly132-Ser288) |
Synonym: | Fibroblast Growth Factor 2, FGF-2, Basic Fibroblast Growth Factor, bFGF, Heparin-Binding Growth Factor 2, HBGF-2, FGF2, FGFB |
Description: | Recombinant Human Fibroblast growth factor 2 Protein is produced in E.coli and the target gene encoding Gly132-Ser288 is expressed. |
Source: | E. coli |
AA Sequence: | GTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQ LQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALK RTGQYKLGSKTGPGQKAILFLPMSAKS |
Purity: | Greater than 95% as determined by reducing SDS-PAGE. |
Endotoxin: | < 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Bioactivity: | The ED50 for this effect is less than 0.6 ng/mL, measured in a cell proliferation assay using BALB/c 3T3 cells. |
Formulation: | Recombinant Human Fibroblast Growth Factor 2/FGF2/FGFb Protein (Gly132-Ser288)was lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 7.5. |
Stability: | Recombinant Human Fibroblast Growth Factor 2/FGF2/FGFb Protein (Gly132-Ser288)is stable for up to 1 year from date of receipt at -70℃. |
Reconstitution: | Please centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Usage: | For Lab Research Use Only |
Storage: | Lyophilized recombinant human FGF2 protein should be stored below -20°C, though stable at room temperature for 3 weeks.Reconstituted recombinant human FGF2 protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted recombinant human FGF2 protein samples are stable below -20°C for 3 months. |