Cat#: | RP-6412H |
Product Name: | Recombinant Human VEGF (121a.a.) Protein, HEK |
Synonym: | Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609. |
Description: | Recombinant Human VEGF 121 amino acids, produced in HEK cells is a glycosylated 37kDa homodimer and 50kDa homotrimer. The VEGF is purified by proprietary chromatographic techniques. |
Source: | HEK. |
Purity: | Greater than 95% as obsereved by SDS-PAGE. |
Bioactivity: | The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells), the ED50 is 3ng/ml. |
Formulation: | VEGF was lyophilized from a 0.2µm filtered solution containing 1xPBS. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized VEGF in sterile PBS containing 0.1% endotoxin-free recombinant HSA. |
Storage: | Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |