Cat#: | RP-6204H |
Product Name: | Recombinant Human TPO Protein, HEK |
Synonym: | Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO. |
Description: | Thrombopoietin Protein produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation. The TPO is purified by proprietary chromatographic techniques. |
Source: | HEK. |
Purity: | Greater than 95% as obsereved by SDS-PAGE. |
Bioactivity: | The activity was determined by the dose-dependent stimulation of the proliferation of MO7e cells, the EC50 is 3.8ng/ml. |
Formulation: | The TPO protein was filtered (0.2µm) and lyophilized from 0.73mg/ml in 1xPBS. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized Thrombopoietin in sterile PBS containing 0.1% endotoxin-free recombinant HSA. |
Storage: | Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |