Cat#:RP-4146H;Product Name:Recombinant Human Insulin Protein, Yeast;Synonym:Insulin;Description:Insulin Protein produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Zinc content was found to be 0.4%. Insulin is purified by proprietary chromatographic techniques.;Source:Saccharomyces cerevisiae.;Purity:Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:27.5 units/mg. Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts). The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the;Formulation:The protein was lyophilized from 50mM Sodium Chloride solution.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Insulin in PBS pH-7.5.;Storage:Lyophilized Insulin although stable at room temperature, should be stored at 4°C. Upon reconstitution Insulin should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;