Cat#:EIA-730C;Product Name:Chicken IgG ELISA Kit;Size:96T;Sample:serum, plasma, or other biological fluids;Intended use:RUO;Synonym:IgG; Ig gamma 1 chain C region; IGHG1; Immunoglobin heavy constant gamma 1; Immunoglobulin G;Background:Avian antibodies have been recognized for decades and offer many advantages to mammalian antibodies in research and diagnostics (Larsson, 1993). The only avian species in which antibodies are highly defined and easily accessible are the chicken. In mammals, immunoglobulins are classified into five groups: IgA, IgD, IgE, IgG and IgG. Chickens possess three principle classes of immunoglobulins: IgA, IgY (IgG) and IgM (Lebacq-Verheyden, 1974). Moreover, the structures of the corresponding immunoglobulins are significantly different, namely in the number of constant domains within the heavy chains. While avian IgY (IgG) is comprised of four constant domains, mammalian IgG contains only three (Hodek, 2003). Chicken IgG is the major serum antibody but is also actively transported to the egg in a manner similar to the placental transfer of IgG in mammals (Larsson 1993 – Review-Chicken Antibodies). The average concentration of IgY (IgG) in chicken serum (and also yolk) is 6 to 13 mg/ml. IgY (IgG) is also found mainly in the egg yoke but is also present in the egg white but in low amounts.;Species Reactivity:Chicken;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;