Cat#:EIA-731C;Product Name:Chicken IgA ELISA Kit;Size:96T;Sample:serum, plasma, or other biological fluids;Intended use:RUO;Synonym:IgA; Immunoglobulin A; GAMMA-A-GLOBULIN; Ig alpha 1 chain C region; Ig alpha 2 chain C region; IgA Heavy Chain; IGHA1; IGHA2; Immunoglobulin heavy constant alpha 1; Immunoglobulin heavy constant alpha 2 A2m marker; Immunoglobulin heavy constant alpha 2;Background:Avian antibodies have been recognized for decades and offer many advantages to mammalian antibodies in research and diagnostics. The only avian species in which antibodies are highly defined and easily accessible are the chicken. In mammals, immunoglobulins are classified into five groups: IgA, IgD, IgE, IgG and IgA. Chickens possess three principle classes of immunoglobulins: IgM, IgY (IgG) and IgA (Lebacq-Verheyden, 1974). Moreover, the structures of the corresponding immunoglobulins are significantly different, namely in the number of constant domains within the heavy chains. While avian IgY (IgG) is comprised of four constant domains, mammalian IgG contains only three (Hodek, 2003). The average concentration of IgA in chicken serum is about 0.33 mg/ml and represents less than 4% of the total immunoglobulins. However, chicken IgA is the predominate immunoglobulin in chicken bile and intestinal secretions (Lebacq-Verheyden, 1974). IgA is also known to be transferred with other proteins in the oviduct into the egg white but in very low amounts.;Species Reactivity:Chicken;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;