Cat#: | PA-2760F |
Product Name: | Mouse Anti-Gentamicin Antibody |
Synonym: | Gentamicin; GENTAMICIN; GENTAMYCIN; GENTAMYCINE; GentamysinsolutionforBiochemistry; Gentacycol; Gentavet; GENTAMICINUM; Refobacin tm; Uromycine; Gentamicin (base and/or unspecified salts); Lyramycin |
Gene Introduction: | This monoclonal antibody was generated from Balb/c mice immunized with gentamicin-KLH conjugate. Its antigen-binding ability was shown by its specific recognition of gentamicin that has been conjugated in BSA by ELISA and immune-gold based lateral flow assay, respectively; the recognition is inhibited in the presence of free gentamicin in the test samples. No significant cross-reaction to other unrelated antibiotics tested (including Penicillin G, Ampicilline, Tetracycline, kanamycine and neomycin) was detected by competitive ELISA. This antibody can be used as a detecting reagent in assays designed to quantitatively or semi-quantitatively measure gentamicin, such as competitive ELISA or immunochromatography-based lateral flow.In addition, for ELISA in combination with the gentamicin-BSA conjugate as coating antigen and HRP-conjugated goat-anti-mouse IgG as secondary antibody, this antibody at as lower as 5 ng/ml (i.e., 1: 200, 000 dilutions of 1 mg/ml) was shown to still give decent OD readings after addition of relevant substrates. However, the optimal working dilutions for each specific application should be determined by the user empirically. |
Description: | Mouse Anti-Gentamicin Monoclonal Antibody |
Formulation: | Liquid Solution |
Host Species: | Mouse |
Species Reactivity: | N/A |
Clone#: | G16-43 |
Isotype: | IgG1a |
Application: | ELISA, LF |
Usage: | For Lab Research Use Only |
Storage: | Store antibody products at 2-8°C. For long term storage, aliquot and freeze at -20°C. Avoid repeated freeze/thaw cycles |