Cat#:RP-2364R;Product Name:Recombinant Rat M CSF Protein;Synonym:Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csf1, Csfm.;Description:Macrophage Colony Stimulating Factor Rat Recombinant produced in E.coli is a non-glycosylated homodimer, containing 2 x 155 amino acids and having a total molecular mass of 36.2 kDa. MCSF is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP.;Purity:>95.0% as determined by SDS-PAGE.;Bioactivity:The activity is determined by dose-dependent induction of M-NFS-60 cell proliferation and is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5x105 units/mg.;Formulation:Lyophilized from a sterile filtered aqueous solution containing 10mM Na3PO4, pH 7.5.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized MCSF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Macrophage Colony Stimulating Factor Rat Recombinant produced in E.coli is a non-glycosylated homodimer, containing 2 x 155 amino acids and having a total molecular mass of 36.2 kDa. MCSF is purified by proprietary chromatographic techniques.
The activity is determined by dose-dependent induction of M-NFS-60 cell proliferation and is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5x105 units/mg.
Formulation:
Lyophilized from a sterile filtered aqueous solution containing 10mM Na3PO4, pH 7.5.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized MCSF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.