Cat#:RP-2318R;Product Name:Recombinant Rat GM CSF Protein;Synonym:CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.;Description:Granulocyte Macrophage Colony Stimulating Factor Rat Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton. GM-CSF Rat Recombinant is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.;Purity:>95.0% as determined by: (a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE.;Bioactivity:The ED50 range = 0.001-0.01ng/ml corresponding to a specific activity of 100,000,000-1,000,000,000IU/mg, determined by the dose dependent proliferation of murine MC/9 cells.;Formulation:GM-CSF Rat was lyophilized with no additives.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;