Cat#:RP-2315R;Product Name:Recombinant Rat GDNF Protein;Synonym:GDNF; glial cell derived neurotrophic factor; HFB1-GDNF; glial cell line-derived neurotrophic factor; ATF; HSCR3; astrocyte-derived trophic factor; ATF1; ATF2;Description:Recombinant Rat Glial derived Neurotrophic Factor / GDNF protein expressed in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 134 amino acids and having a total molecular mass of 29.8 kDa. The recombinant rat GDNF protein is purified by our unique chromatographic techniques.;Source:E.coli;AA Sequence:SPDKQAALP RRENRNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCESAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDNLVY HILRKHSAKR CGCI ;Purity:>98.0% as determined by HPLC analysis and by SDS-PAGE.;Bioactivity:Recombinant rat GDNF protein has full biological activity when compared to standards. The ED50, determined by a cell proliferation assay using rat C6 cells, is less than 0.2ng/ml corresponding to a specific activity of more than 5,000,000IU/mg.;Formulation:Recombinant rat GDNF protein was lyophilized from a sterile solution containing 1xPBS, pH 7.4.;Stability:Recombinant rat GDNF Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized recombinant Glial Derived Neurotrophic Factor protein in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant rat Glial-derived Neurotrophic Factor / GDNF protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant rat GDNF protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.;References:Lin LF, Doherty DH, Lile JD, Bektesh S, Collins F (May 1993). "GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons". Science. 260 (5111): 1130–2.;
Recombinant Rat Glial derived Neurotrophic Factor / GDNF protein expressed in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 134 amino acids and having a total molecular mass of 29.8 kDa. The recombinant rat GDNF protein is purified by our unique chromatographic techniques.
>98.0% as determined by HPLC analysis and by SDS-PAGE.
Bioactivity:
Recombinant rat GDNF protein has full biological activity when compared to standards. The ED50, determined by a cell proliferation assay using rat C6 cells, is less than 0.2ng/ml corresponding to a specific activity of more than 5,000,000IU/mg.
Formulation:
Recombinant rat GDNF protein was lyophilized from a sterile solution containing 1xPBS, pH 7.4.
Stability:
Recombinant rat GDNF Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Glial Derived Neurotrophic Factor protein in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized recombinant rat Glial-derived Neurotrophic Factor / GDNF protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant rat GDNF protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
References:
Lin LF, Doherty DH, Lile JD, Bektesh S, Collins F (May 1993). "GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons". Science. 260 (5111): 1130–2.
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