Cat#:RP-2395R;Product Name:Recombinant Rat CCL17 / TARC Protein;Synonym:Thymus and activation-regulated chemokine, CCL17, SCYA17, TARC.;Description:Recombinant rat CCL17 / TARC protein expressed in E.coli is a non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 8.1kDa. The recombinant rat TARC protein is purified using our unique chromatographic techniques.;Source:E.coli;AA Sequence:ARATNVGREC CLDYFKGAIP IRKLVTWFRT SVECPKDAIV FETVQGRLIC TDPKDKHVKK AIRHLKNQRL.;Purity:>97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The recombinant rat CCL17 protein was determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 1.0-10.0 ng/ml.;Formulation:The recombinant rat CCL17 protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH7.4.;Stability:Recombinant Human CCL17 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized rat TARC protein in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant rat CCL17 / TARC protein although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution recombinant rat CCL17 protein should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.;References:Tumor-associated macrophage or chemokine ligand CCL17 positively regulates the tumorigenesis of hepatocellular carcinoma. Zhu F, et al. Med Oncol, 2016 Feb. PMID 26781124;
Thymus and activation-regulated chemokine, CCL17, SCYA17, TARC.
Description:
Recombinant rat CCL17 / TARC protein expressed in E.coli is a non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 8.1kDa. The recombinant rat TARC protein is purified using our unique chromatographic techniques.
>97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bioactivity:
The recombinant rat CCL17 protein was determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 1.0-10.0 ng/ml.
Formulation:
The recombinant rat CCL17 protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH7.4.
Stability:
Recombinant Human CCL17 Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized rat TARC protein in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized recombinant rat CCL17 / TARC protein although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution recombinant rat CCL17 protein should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
References:
Tumor-associated macrophage or chemokine ligand CCL17 positively regulates the tumorigenesis of hepatocellular carcinoma. Zhu F, et al. Med Oncol, 2016 Feb. PMID 26781124