Cat#:RP-RANKL-01M;Product Name:Recombinant Mouse RANKL / OPGL / TNFSF11 / CD254 Protein(Fc Tag);Synonym:RANKL, OPGL,TNFSF11,CD254,hRANKL2;Background:The full name of RANKL gene is receptor activator of nuclear factor-κB ligand gene. This gene, also known as TNFSF11 gene, encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. RANKL has been identified to affect the immune system and control bone regeneration and remodeling.;Description:A DNA sequence encoding the extracellular domain of mouse RANKL/TNFSF11 (BAA97257.1) (Arg 72-Asp 316) fused with the Fc Tag at the N-terminus was produced in Human cells. The RANKL mouse recombinant protein was purified by our unique purification techniques.;Source:Human Cells;Predicted N Terminal:Glu ;Molecular Characterization:The recombinant mouse RANKL/TNFSF11/Fc is a disulfide-linked homodimer. The reduced monomer comprises 505 amino acids and has a calculated molecular mass of 56 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.;Purity:Greater than 87 % as determined by SDS-PAGE;Endotoxin:< 1.0 EU per μg of the mouse rankl protein as determined by the LAL method;Bioactivity:Immobilized mouse Fc-rankl at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His, The EC50 of biotinylated human TNFRSF11B-His is 0.07-0.17 μg/ml.;Formulation:Recombinant mouse RANKL protein was lyophilized from sterile PBS, pH 7.4;Stability:Recombinant mouse RANKL Proteins are stable for up to 1 year from date of receipt at -70℃;Host Species:Mouse;Storage:Store recombinant mouse RANKL protein under sterile conditions at -20℃ to -80℃. It is recommended that the mouse rankl protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.;References:1. Nakashima T, et al. (2003) RANKL and RANK as novel therapeutic targets for arthritis. Curr Opin Rheumatol. 15(3): 280-7. 2.Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50. ;
The full name of RANKL gene is receptor activator of nuclear factor-κB ligand gene. This gene, also known as TNFSF11 gene, encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. RANKL has been identified to affect the immune system and control bone regeneration and remodeling.
Description:
A DNA sequence encoding the extracellular domain of mouse RANKL/TNFSF11 (BAA97257.1) (Arg 72-Asp 316) fused with the Fc Tag at the N-terminus was produced in Human cells. The RANKL mouse recombinant protein was purified by our unique purification techniques.
Source:
Human Cells
Predicted N Terminal:
Glu
Molecular Characterization:
The recombinant mouse RANKL/TNFSF11/Fc is a disulfide-linked homodimer. The reduced monomer comprises 505 amino acids and has a calculated molecular mass of 56 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
Purity:
Greater than 87 % as determined by SDS-PAGE
Endotoxin:
< 1.0 EU per μg of the mouse rankl protein as determined by the LAL method
Bioactivity:
Immobilized mouse Fc-rankl at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His, The EC50 of biotinylated human TNFRSF11B-His is 0.07-0.17 μg/ml.
Formulation:
Recombinant mouse RANKL protein was lyophilized from sterile PBS, pH 7.4
Stability:
Recombinant mouse RANKL Proteins are stable for up to 1 year from date of receipt at -70℃
Host Species:
Mouse
Storage:
Store recombinant mouse RANKL protein under sterile conditions at -20℃ to -80℃. It is recommended that the mouse rankl protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
References:
1. Nakashima T, et al. (2003) RANKL and RANK as novel therapeutic targets for arthritis. Curr Opin Rheumatol. 15(3): 280-7. 2.Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50.