Cat#:RP-1864M;Product Name:Recombinant Mouse Leptin Protein;Synonym:OB Protein, Obesity Protein, OBS, Obesity factor.;Description:Leptin Mouse Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.2 kDa. The Leptin is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC.;Purity:Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:Biological activity of Mouse Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gram body weight for a period of 14 days. Significant;Formulation:The mouse Leptin was lyophilized from a concentrated (1mg/ml) solution containing 0.1% TFA.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;