Cat#:RP-1807M;Product Name:Recombinant Mouse IL12 Protein;Synonym:NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.;Description:Interleukin-12 Mouse Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 506 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. The IL-12 is purified by proprietary chromatographic techniques.;Source:HEK-293 Cells.;AA Sequence:p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA. p40 Subunit: MWELEKDVYV VEVD;Purity:Greater than 95% as determined by SDS-PAGE.;Bioactivity:IL-12 Mouse has full biological activity when compared to standards. The ED50 is determined by the dose-dependent induced expression of CD25 on CD8+ mouse T cells is 10-20 ng/ml corresponding to a specific activity of 50,000-100,000units/mg.;Formulation:The protein was lyophilized from 0.5xPBS, pH 7.5.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized murine IL12 in sterile distilled pyrogen free water at 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized mouse IL-12 although stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;