Cat#: | RP-1527M |
Product Name: | Recombinant Mouse Smad5 Protein (His & GST Tag) |
Synonym: | 1110051M15Rik,AI451355,Dwf-C,Madh5,MusMLP |
Description: | A DNA sequence encoding the mouse SMAD5 (P97454) (Thr2-Ser465) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Source: | Baculovirus-Insect Cells |
Predicted N Terminal: | Met |
Molecular Characterization: | The recombinant mouse SMAD5/GST chimera consists of 701 amino acids and has a calculated molecular mass of 79.9 kDa. The recombinant protein migrates as an approximately 75-88 kDa band in SDS-PAGE under reducing conditions. |
Purity: | Greater than 95 % as determined by SDS-PAGE |
Endotoxin: | < 1.0 EU per ug of the protein as determined by the LAL method |
Bioactivity: | Please contact us for detailed information |
Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | Please see related COA for specific instructions. |
Storage: | Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃). |