Cat#:RP-1796M;Product Name:Recombinant Mouse IGF1 Protein;Synonym:Insulin like growth factor 1, IBP1, Igf-1; Igf-I; C730016P09Rik, Somatomedin C,IGF1, IGF-I, IGFIA;Description:Recombinant mouse Insulin-Like Growth Factor I /IGF1 protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton. Recombinant mouse IGF-I protein is purified by our unique chromatographic techniques.;Source:E.coli;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu.;Purity:Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 1.0 ng/ml, corresponding to a specific activity of 1MU/mg.;Formulation:The recombinant mouse IGF1 protein was lyophilized with no additives.;Stability:Recombinant mouse IGF1 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized mouse IGF1 protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant mouse IGF1 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant mouse IGF1 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.;References:The effects of IGF-1 on mouse spermatogenesis using an organ culture method. Yao J, et al. Biochem Biophys Res Commun, 2017 Sep 23. ;