Cat#:RP-1822M;Product Name:Recombinant Mouse IL21 Protein;Synonym:Interleukin-21, IL-21, Il21.;Description:Interleukin-21 Mouse Recombinant produced in E.coli is a single, non-glycosilated polypeptide chain containing 130 amino acids and having a total molecular mass of 15kDa. The Murine IL-21 is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Ser-Ser.;Purity:Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The ED50=10 -50 ng/ml, determined by the cell proliferation assay of modified Ba/F3 cells.;Formulation:Mouse IL-21 was lyophilized from 20mM NaHCO3, pH 8.5.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Mouse IL21 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Mouse IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse IL21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;