Cat#: | RP-1665M |
Product Name: | Recombinant Mouse b NGF Protein |
Synonym: | Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428. |
Description: | Recombinant Mouse b-NGF produced in E.coli is a noncovalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical chains of 120 amino acids each and having a molecular mass of 13,471 Dalton each. The Recombinant Mouse-beta-NGF is purified by advanced biology purification technology. |
Source: | E.coli |
AA Sequence: | MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G. |
Purity: | Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE . |
Bioactivity: | The Mouse b-NGF activity was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1, the ED50 for this effect is 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 units/mg. |
Formulation: | The Recombinant Mouse b-NGF was lyophilized without additives. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized Murine NGF-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage: | Lyophilized Mouse Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Murine NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. |