Cat#:RP-3113HAT;Product Name:Recombinant Human Tumor Necrosis Factor-alpha Protein;Synonym:Tumor necrosis factor alpha;Description:The human recombinant TNF-alpha was produced in E. coli. This recombinant protein was purified using our unique purification methods. Additional amino acid, methionine, was attached at N Terminus of the protein.;Source:E.coli;AA Sequence:77-233 aa;Molecular Characterization:17.5 kDa;Purity:Greater than 95% by SDS-PAGE;Endotoxin:< 1.0 EU per ug of protein as determined by LAL method.;Bioactivity:Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less or equal to 0.2 ng/ml.;Formulation:Liquid Solution;Stability:Recombinant Human Tumor Necrosis Factor-alpha Protein was stable up to 1 year from date of receipt at -70℃;Concentration:1mg/mL;Storage Buffer:Recombinant Human Tumor Necrosis Factor-alpha protein was supplied in PBS, pH 7.4;Storage:Recombinant Human Tumor Necrosis Factor-alpha Protein can be stored at +4℃for 1-2 weeks. It is recommended that the recombinant protein be aliquoted for optimal storage at -20 or -70℃. Please avoid repeated freeze-thaw cycles.;Usage:For Lab Research Use Only;