Cat#: | RP-6411H |
Product Name: | Recombinant Human VEGF (121 a.a.) Protein, Sf9 |
Synonym: | Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609. |
Description: | Vascular Endothelial Growth Factor-121 Protein produced in insect cells as an 18kDa homodimer, is a glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of approximately 36kDa. VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates. The VEGF-121 is purified by proprietary chromatographic techniques. |
Source: | Sf9, Insect Cells. |
AA Sequence: | APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPS CVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN KCECRPKKDRARQEKCDKPRR. |
Purity: | Greater than 95.0% as determined by SDS-PAGE. |
Bioactivity: | Measured in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 2-10ng/ml. |
Formulation: | The protein was lyophilized from a solution containing 50mM acetic acid. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | The lyophilized VEGF121 should be reconstituted in 50mM acetic acid to a concentration not lower than 50µg/ml. |
Storage: | Lyophilized Vascular Endothelial Growth Factor 121 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-121 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |