Cat#:RP-6135H;Product Name:Recombinant Human TNF a Protein(His Tag);Synonym:TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.;Description:Tumor Necrosis Factor-a Protein His produced in E.coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids fragment (77-233) and having a molecular mass of 18.3kDa with an amino-terminal hexahistidine tag. The TNF-alpha His is purified by standard chromatographic techniques.;Source:E.coli;Purity:Greater than 97.0% as determined by SDS-PAGE.;Bioactivity:EC50 0.200-0.120ng/ml measured in a metabolic inhibitor actinomycin D assay using L-cells (ATCC CCL-1).;Formulation:TNF-a His is supplied in 1xPBS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized TNF-α in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;