Cat#:RP-3988H;Product Name:Recombinant Human IGF1 Gilthead Seabream Protein;Synonym:Somatomedin C, IGF-I, IGFI.;Description:Recombinant human Insulin-Like Growth Factor-I Gilthead Seabream protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7545.4 Dalton, the predicted pI=7.72.Recombinant human IGF1 protein is purified by our unique chromatographic techniques.;Source:E.coli;AA Sequence:The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ser-Pro-Glu-Thr-Leu-Cys-Gly-Ala-Glu.;Purity:Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain;Formulation:The recombinant human IGF1 Gilthead Seabream Protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.;Stability:Recombinant human IGF1 Gilthead Seabream Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized recombinant human IGF1 protein in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant human IGF1 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human IGF1 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;