Cat#:RP-3217H;Product Name:Recombinant Human FGF21 Protein(His Tag);Synonym:Fibroblast growth factor 21, FGF-21.;Description:Fibroblast Growth Factor -21 Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 202 amino acids (29-209) and having a molecular mass of 21.6 kDa (molecular weight on SDS-PAGE will appear higher). The FGF-21 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVKTSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS.;Purity:Greater than 90.0% as determined by SDS-PAGE.;Bioactivity:Measured in a cell proliferation assay using NIH-3T3 cells. The ED50 for this effect is <12ng/ml, which corresponds to > 83,000 units/ml.;Formulation:The FGF-21 His tag protein (1mg/ml) solution in 20mM Tris-HCl buffer pH-8 and 10% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.;