Cat#:RP-0146H;Product Name:Recombinant Human BMPRIA / ALK-3 / CD292 Protein (His & Fc Tag);Synonym:10q23del,ACVRLK3,ALK3,CD292,SKR5;Description:Recombinant Human BMPRIA protein was produced in human cells. A DNA sequence encoding the extracellular domain (Met 1-Arg 152) of human ALK3 (NP_004320.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. The recombinant human BMR1A protein is purified by our unique chromatographic techniques.;Source:Human Cells;Predicted N Terminal:Gln 24;Molecular Characterization:The recombinant human ALK-3 protein/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 376 amino acids and has a predicted molecular mass of 42 kDa. Under reducing conditions, the apparent MW of ALK-3 monomer is approximately 56 kDa.;Purity:Greater than 97 % as determined by SDS-PAGE;Endotoxin:< 1.0 EU per ug of the Recombinant Human BMPRIA protein as determined by the LAL method;Bioactivity:Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.1-0.3 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.;Formulation:Recombinant Human BMPRIA protein was lyophilized from sterile PBS, pH 7.4;Stability:Recombinant Human BMPRIA Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:Please see related COA for specific instructions.;Storage:Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃).;References:Zielins ER, et al. Enrichment of Adipose-Derived Stromal Cells for BMPR1A Facilitates Enhanced Adipogenesis.Tissue Eng Part A, 2016 Feb. PMID 26585335 ;