Cat#:EIA-435C;Product Name:Human Oestradiol ELISA Kit;Size:96T;Sample:human serum or plasma;Intended use:in vitro diagnosis;Synonym:Oestradiol; Almediol; alpha-Oestradiol; Altrad; Amnestrogen; Aquadiol; Bardiol; beta-Oestradiol; cis-Oestradiol; Climara; component of Menrium; Corpagen; d-3,17-.beta.-Estradiol; d-3,17-.beta.-Oestradiol; d-3,17-beta-estradiol; D-3,17beta-Estradiol; d-3,17-beta-oestradiol; D-3,17beta-Oestradiol; D-Estradiol; Dihydrofollicular hormone; Dihydrofollicularhormone; 3,17-beta-estradiol; 3,17beta-Estradiol; 3,17-beta-Oestradiol; alpha.-Oestradiol; beta.-Oestradiol;Background:Oestradiol (E2) is a C18 steroid human hormone with a phenolic A ring . This steroid hormone has a molecular weight of 272.4 daltons. It is the most potent natural Oestrogen, produced mainly by the ovary, placenta, and in smaller amounts by the adrenal cortex, and the male testes. Oestradiol (E2) is secreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG). To a lesser extent it is bound to other serum proteins such as albumin. Only a tiny fraction circulates as free hormone or in the conjugated form. Oestrogenic activity is affected via Oestradiol-receptor complexes which trigger the appropriate response at the nuclear level in the target sites. These sites include the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent, the liver and skin. In non-pregnant women with normal menstrual cycles, Oestradiol sectretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. The rising Oestradiol concentration is understood to exert a positive feedback influence at the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone (FSH) and luteinising hormone (LH), which are essential for follicle maturation and ovulation, respectively. Following ovulation, Oestradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of Oestradiol in the luteal phase. During pregnancy, maternal serum Oestradiol levels increase considerably to well above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy. Serum Oestradiol measurements are a valuable index in evaluating a variety of menstrual dysfunctions such as precocious or delayed puberty in girls and primary and secondary amenorrhea and menopause. Oestradiol levels have been reported to be increased in patients with feminising syndromes, gynaecomastia and testicular tumours. In cases of infertility, serum Oestradiol measurements are useful for monitoring induction of ovulation following treatment with, for example, clomiphene citrate, LH-releasing hormone (LH-RH), or exogenous gonadotropins. During ovarian hyperstimulation for in vitro fertilisation (IVF), serum Oestradiol concentrations are usually monitored daily for optimal timing of human chorionic gonadotropin (hCG) administration and oocyte collection.;Species Reactivity:Human;Application:quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Competitive ELISA;