Cat#:EIA-706C;Product Name:Diphtheria Toxoid IgG ELISA Kit;Size:96 T;Sample:serum and plasma;Intended use:in-vitro diagnostic;Synonym:Corynebacterium diphtheriae toxoid; Diphtheria Toxin; tox; Diphtheria Toxoid;Background:Diphtheria is a bacterial infectious disease which appears predominantly during the childhood. The disease leads particularly to an inflammation of the pharynx, larynx and nasal mucosa. Additionally, bacterial toxins cause via long-distance effect damages of the heart, circulation and CNS. Only the toxigenic strains are pathogenic. The etiologic agent is the Corynebacterium diphtheriae. These gram-positive bacteria prefer a microaerophil to anaerobe environment. Its pathogenicity is based on the secretion of an exotoxin that is circulating in the blood and effecting the heart muscle, kidneys and CNS. The Diphtheria toxoid will be produced by lysogenic strains. Depending on the stage of disease, the three types 'slight, middle and serious' can be distinguished. The natural source of infection is the sick individual, whereas a carrier not absolutely shows symptoms. The infection is spread both through the aerial-droplet route and rarely by milk or smear infection. The appearance of Diphtheria shows a seasonal prevalence with the greatest incidence in winter. Especially non-vaccinated children will be infected. The incubation time is depending on the number of invasive germs. The place of infection is the mucosa of the respiratory tract, where an acute local infection is developing. The secreted toxin leads to a superficial inflammation of the mucosa associated with the formation of a brown film (pseudo-membrane) upon it, consisting of bacteria, necrotic epithelial cells, fibrin, red and white cells. From this local inflammation, the toxin reaches other organs by using the blood and lymphatic circulation. Here it may cause severe damages. The grade of disease depends on the immunostate of the child. Usually, a limited Diphtheria arises, whereas in case of an immunosuppression, a severe Diphtheria is observed. As a result of this disease course, patients may die. In most cases children will be vaccinated (e.g. DTP = Diphtheria-Tetanus-Pertussis) after the third month of life. The state of immunity can be monitored by determining the antitoxin IgG.;Species Reactivity:Bacteria;Application:quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:I-ELISA;