Cat#:EIA-728C;Product Name:Chicken IL16 ELISA Kit;Size:96T;Sample:cell culture supernatants;Intended use:RUO;Synonym:interleukin 16 (lymphocyte chemoattractant factor); IL16; interleukin 16; interleukin-16;Background:Interleukin 16 (IL-16) is a pleiotropic cytokine that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4, and has effects on mixed lymphocyte reaction and inhibition of HIV viral replication. Because this cytokine was discovered in 1982 for its T lymphocyte chemotactic activity, it was named “Lymphocyte Chemoattractant Factor (LCF)” until it was designated interleukin-16 in 1995. The product of this gene is generated as a precursor molecule, pro-IL-16, which is then processed to yield two functional proteins. IL-16 forms homotetramers and this structure is required for its bioactivity. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. IL-16 is released by a variety of immune (T cells, esinophils, and dendritic cells) and non-immune (fibroblasts, epithelial, and neurona) cells (Center et al., 1997; Cruikshank et al., 2000). In the non-diseased state, IL-16 mRNA is almost exclusively expressed on lymphatic tissue, and high levels in T cells. During inflammation, IL-16 is synthesized in a number of other tissues (Smith and Humphries, 2009). The sequence, structure, and function of IL-16 are highly conserved in all species examined. Thus far, all species of IL-16 tested induce similar bioactivities on CD4+ T cells. Chicken IL-6 has been cloned by Min et al. (2004) and shows 86% sequence identity to duct pro-IL-16, and 49-52% to various mammalian homologues. The recombinant chicken IL-16 showed chemoattractive activity to splenic lymphocytes.;Species Reactivity:Chicken;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;