Cat#:TN5-001Z;Product Name:Novo™ Tn5 Transposase;Size:25U, 125U;Intended use:• Library construction for second-generation sequencing. • In vitro transgenic experiment.;Synonym:Transposase (Tnp) Tn5, Tn5 Transposase;Description:Novo™ Tn5 Transposase is a hyperactive form of Tn5 transposase. Tn5 Transposase can recognize inside, outside and mosaic end sequences of Tn5 transposon. This product can be used to randomly insert Tn5 transposon into target DNA. We offer high quality Tn5 transposase for in vitro transgenic (foreign gene integration into host cells) and library construction for second-generation sequencing.;Source:A E. coli strain containing the sequence of the Tn5.;Concentration:1,000 U/mL;Storage Buffer:50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol (V/V) ;Storage:The Tn5 Transposase is shipped using dry ice. Once receiving, please store Tn5 Transposase at -20℃.Avoid repeated freeze-thaw cycles. ;Components:Tn5 Transposase(1 U/μl); 5× LM Buffer; 10×TPS Buffer;Unit Definition:One unit Tn5 transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition seqence in 1 hour at 37°C.;References:Picelli S, etal. Tn5 transposase and tagmentation procedures for massively scaled sequencing projects.2014 Dec;24(12):2033-40. ;