Cat#:RP-5912H;Product Name:Recombinant Streptavidin Protein;Synonym:Streptavidin;Background:Streptavidin is a 52.8 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7 or vitamin H).Streptavidin is used extensively in molecular biology and bionanotechnology. The strong streptavidin-biotin bond can be used to attach various biomolecules to one another or onto a solid support.Pretargeted immunotherapy uses streptavidin conjugated to a monoclonal antibody against cancer cell-specific antigens followed by an injection of radiolabelled biotin to deliver the radiation only to the cancerous cell.;Description:Streptavidin is a protein produced by Streptomyces avidinii and isolated by purification from fermentation broth. The pure, homogeneous protein shows predominantly one single band in SDS PAGE. Streptavidin consists of 4 identical subunits, each bearing an active binding site for biotin. Streptavidin has a molecular weight of 55kDa.;Source:Bacterium Streptomyces avidinii.;Formulation:Recombinant Streptavidin protein was lyophilized (1mg/ml) in 50mM NaCl, pH 9.0.;Stability:Recombinant Streptavidin Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:Gives a clear solution at 5mg/ml in 0.1M NaCl.;Storage:Streptavidin although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter. Please prevent freeze-thaw cycles.;References:Weber, P. C. (1989). "Structural Origins of High-Affinity Biotin Binding to Streptavidin". Science. 243 (4887): 85–8.;