Cat#: | RP-273A |
Product Name: | Recombinant Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein |
Synonym: | gB |
Description: | A DNA sequence encoding the extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907) of human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') was expressed, fused with five additional amino acids (DDDDK) at the C-terminus. |
Source: | Human Cells |
Predicted N Terminal: | Ser 25 |
Molecular Characterization: | The recombinant glycoprotein B of human CMV consists of 813 amino acids and has a predicted molecular mass of 92 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB is approximately 130-140 kDa due to glycosylation. |
Purity: | Greater than 90 % as determined by SDS-PAGE |
Endotoxin: | < 1.0 EU per ug of the protein as determined by the LAL method |
Bioactivity: | Please contact us for detailed information |
Formulation: | Lyophilized from sterile Tris 50mM, NaCl 100mM, pH 8.0 |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | Please see related COA for specific instructions. |
Storage: | Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃). |