Cat#: | RP-267A |
Product Name: | Recombinant Human Enterovirus 71 VP0 Protein (His & GST Tag) |
Synonym: | VP0,VP4-VP2 |
Description: | A DNA sequence encoding the amino acids (Met 1-Gln 323) of human enterovirus 71 genome polyprotein (Q66478-1), corresponding to the protein VP0 precusor, a component of immature procapsids, was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Source: | Baculovirus-Insect Cells |
Predicted N Terminal: | Met |
Molecular Characterization: | The recombinant human enterovirus 71 protein VP0/GST chimera consists of 560 amino acids and has a calculated molecular mass of 63 kDa. It migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions. |
Purity: | Greater than 72 % as determined by SDS-PAGE |
Endotoxin: | < 1.0 EU per ug of the protein as determined by the LAL method |
Bioactivity: | Please contact us for detailed information |
Formulation: | Lyophilized from sterile 50mM Tris, 100mM NaCl, 2mM GSH, 0.5mM PMSF, pH 8.0 |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | Please see related COA for specific instructions. |
Storage: | Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃). |