Cat#: | RP-251A |
Product Name: | Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (ECD, aa 1-1297, His Tag) |
Synonym: | S |
Description: | A DNA sequence encoding the extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012)( AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus. |
Source: | Baculovirus-Insect Cells |
Predicted N Terminal: | Tyr 18 |
Molecular Characterization: | The recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012) comprises 1289 amino acids and has a predicted molecular mass of 142.2 kDa. It migrates as an approximately 157 kDa band in SDS-PAGE under reducing conditions. |
Purity: | Greater than 85 % as determined by SDS-PAGE |
Endotoxin: | < 1.0 EU per ug of the protein as determined by the LAL method |
Bioactivity: | Please contact us for detailed information |
Formulation: | Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | Please see related COA for specific instructions. |
Storage: | Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃). |