Cat#:RP-2328R;Product Name:Recombinant Rat IGF1 Protein;Synonym:Insulin like growth factor 1, IBP1, Somatomedin C,IGF1, IGF-I, IGFIA;Description:Recombinant Rat Insulin-Like Growth Factor I/IGF1 expressed in E.coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa. The recombinant rat IGF1 protein is purified by our unique chromatographic techniques.;Source:E.coli;AA Sequence:GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA.;Purity:>98.0% as determined by SDS-PAGE.;Bioactivity:The ED50 of rat IGF1 protein as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0ng/ml, corresponding to a specific activity of >500,000units/mg.;Formulation:The rat IGF1 protein was lyophilized with a 0.2µm filtered concentrated solution in 20mM PBS, pH 7.0.;Stability:Recombinant Rat IGF1 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized rat IGF1 protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Host Species:Human;Storage:Lyophilized rat IGF1 protein although stable at room temperature for 2-4 weeks, should be stored desiccated below -20°C. Upon reconstitution rat IGF1 protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.;References:Yang X et al. IGF-1 protects retinal ganglion cells from hypoxia-induced apoptosis by activating the Erk-1/2 and Akt pathways. Mol Vis 19:1901-12 (2013);