Cat#:RP-2306R;Product Name:Recombinant Rat FGF1 Protein;Synonym:Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, aFGF, Heparin-binding growth factor 1, HBGF-1, Fgf1, Fgfa, HBGF1.;Description:Rat Recombinant FGF1 protein expressed in E.coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15.9 kDa. The recombinant rat FGF acidic / FGF1 protein is purified by ourunique chromatographic techniques.;Source:E.coli;AA Sequence:MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.;Purity:>98.0% as determined by SDS-PAGE.;Bioactivity:The ED50 of recombinant rat FGF1 protein as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.2ng/ml corresponding to a Specific Activity of 5x106IU/mg.;Formulation:Recombinant rat FGF1 protein was lyophilized at a concentration of 1 mg/ml in 5mM Na2PO4, pH-7.5 and 50mM NaCl.;Stability:Recombinant rat FGF1 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic / FGF1 protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant rat Fibroblast Growth Factor-1 / FGF1 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant rat FGF1 protein should be stored at 4°C between 2-7 days and for future use below -18°C. Please avoid freeze-thaw cycles.;