Cat#:RP-1743M;Product Name:Recombinant Mouse FGF1 Protein;Synonym:HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.;Description: Mouse Recombinant Fibroblast Growth Factor-acidic / FGF1 protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15.8 kDa. The recombinant mouse FGF acidic / FGF1 protein is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:FNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.;Purity:Greater than 96.0% as determined by SDS-PAGE.;Bioactivity:The ED50 of recombinant mouse FGF1 protein as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.2 ng/ml corresponding to a Specific Activity of 5,000,000IU/mg.;Formulation:Recombinant mouse FGF1 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.;Stability:Recombinant mouse FGF1 proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized recombinant mouse Fibroblast Growth Factor-acidic in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant mouse Fibroblast Growth Factor-1 / FGF1 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant mouse FGF1 protein should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;