Cat#: | RP-1971M |
Product Name: | Recombinant Mouse CCL17 / TARC Protein |
Synonym: | C-C motif chemokine 17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CC chemokine TARC, ABCD-2, CCL17, CCL-17, SCYA17, TARC, A-152E5.3, MGC138271, MGC138273. |
Description: | Recombinant mouse CCL17 / TARC protein expressed in E.coli is a non-glycosylated, Polypeptide chain containing 70 amino acids and having a molecular mass of 7.9kDa. The recombinant mouse TARC protein is purified using our unique chromatographic techniques. |
Source: | E.coli |
AA Sequence: | ARATNVGREC CLDYFKGAIP IRKLVSWYKT SVECSRDAIV FLTVQGKLIC ADPKDKHVKK AIRLVKNPRP. |
Purity: | Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Bioactivity: | The ED50 of recombinant mouse CCL17 protein as determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR4 is typically 2-10 ng/ml. |
Formulation: | The recombinant mouse CCL17 protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH7.4. |
Stability: | Recombinant mouse CCL17 Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized mouse TARC protein in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage: | Lyophilized recombinant mouse CCL17 / TARC protein although stable at room temperature for 3 weeks, should be stored desiccated below -20C. Upon reconstitution recombinant mouse CCL17 protein should be stored at 4C between 2-7 days and for future use below -20C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
References: | Tumor-associated macrophage or chemokine ligand CCL17 positively regulates the tumorigenesis of hepatocellular carcinoma. Zhu F, et al. Med Oncol, 2016 Feb. PMID 26781124 |